Optimi Health Corp., is thrilled to announce an innovative research and development partnership with the Kwantlen Polytechnic University Applied Genomics Centre (KPU-AGC). The project, entitled, "Genetic Identification of Psilocybin Containing Mushroom," will utilize KPU-AGC's state-of-the-art genomics technology to unravel the intricate genetic makeup of Optimi's psilocybin-containing mushrooms, potentially paving the way for unprecedented advancements in the field of Psilocybin genetics. Optimi boasts an extensive genetic bank of over 200 psilocybin-containing mushroom strains, making it among the largest globally. The Company aims to produce mushrooms rich in psilocybin and other related tryptamine alkaloids. Through the partnership, Optimi seeks to enhance its DNA characterization abilities, enabling precise species and strain-level identification of mushroom genetics for potential use in clinical trials and authorized medicinal activities. Objectives of the project include: DNA mushroom extraction: the AGC will first extract DNA from 20 mushrooms of the Psilocybe and Panaeolus species cultivated from Optimi's genetics laboratory,
using the cetyltrimethylammonium bromide (CTAB) method. Species Identification by interspaced transcribed spacer (ITS) Sequencing: extracted DNA from the 20 selected samples will undergo ITS sequencing to create a
genotyping protocol for species identification. Strain-level ID by SNPs derived from ddRAD-sequencing: the development of a novel method for strain-level identification, making this the first study that will provide single nucleotide polymorphism (SNP) data for Psilocybe and Panaeolus strains. Optimi and the KPU-AGC are committed to collaboration and innovation in the pursuit of advancing Health Canada approved psychedelic research.