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These automated single-cell total RNA-seq and DNA-seq library preparation kits will provide high sample and cell throughput, less hands-on time, and the ability to capture more information than other available technologies. The Shasta Total RNA-Seq Kit will detect splicing isoforms, gene fusions, and non-polyadenylated RNAs through full-length transcriptome profiling of up to 100,000 single cells per run. Similarly, the Shasta Whole-Genome Amplification Kit will enable novel insights into tumor heterogeneity through copy number variant (CNV) and single nucleotide variant (SNV) analyses of over 1,500 single cells at once.
'Our new kits will break the limits of current methods to enable true biological discovery,' said
Several technological limitations have slowed oncology researchers' ability to discover critical biomarkers. Popular single-cell RNA-seq methods, although high-throughput, lack full-length transcript coverage and detect limited mRNA biotypes-leaving out critical information from precious samples. Even current instrument-free high-throughput single-cell RNA-seq workflows take several days to complete and are limited to mRNA-only readout. Other full-length RNA-seq methods, such as the manual Smart-seq2 protocol, cannot easily be scaled and are time consuming.
Initial methods for single-cell whole-genome amplification have been limited to profiling hundreds of cells at a time. Additionally, current droplet technologies for single-cell DNA-seq, based on targeted panels, fail to capture key genomic mutation events such as chromosome arm-level CNVs, which play pivotal roles in various tumors.
The new technologies were designed as part of an end-to-end solution with the company's single-cell NGS automation platform and Cogent bioinformatics tools, which even beginners can use to create publication-quality figures. Researchers and other professionals wanting details about the launch can sign up to receive priority access to product and technical information.
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