Specimen deformation during SEM observation of food, due to the fat and water content, can occur either by vacuum conditions in the SEM specimen chamber or by thermal effects caused by electron beam irradiation. In order to counteract these effects, the cooling of the specimen before SEM observation is required with a cryo stage or LV cooling holder.
The cryo stage can cool and maintain the specimen at around liquid nitrogen temperature, allowing for observation and analysis of the specimens containing a lot of fats or water (mayonnaise, fresh cream, etc.). However, a conductive coating is often required.
The LV cooling holder allows for precooling the specimen with liquid nitrogen, although does not maintain this temperature. However, the LV cooling holder can be easily combined with low vacuum SEM, allowing for observation and analysis of uncoated samples with a lower water or fat content (raw meats, vegetables, etc.) for a short period of time.
This report presents morphological observation examples of some processed foods, with a cryo stage or LV cooling holder under low vacuum. Specimens used were carbohydrates, frozen foods and meat products.
Please see the PDF file for the additional information.
Another window opens when you click.PDF 998KB
Jeol Ltd. published this content on 17 May 2017 and is solely responsible for the information contained herein. Distributed by Public, unedited and unaltered, on 17 May 2017 01:32:20 UTC.
Original documenthttps://www.jeol.co.jp/en/applications/detail/1384.html
Public permalinkhttp://www.publicnow.com/view/CFEBAA4927BFAD6F951972550D1C3E69932F696A
JEOL Ltd. is a Japan-based company mainly engaged in the manufacture, sale, maintenance, development and research of scientific instruments, semiconductor related equipment, industrial and medical equipment, and related products and parts. It operates through three business segments. Science and Measurement Equipment segment manufactures and sells three types of equipment. Electron optical devices include electron microscopes, peripheral devices and others. Analytical instruments include nuclear magnetic resonance equipment, electron spin resonance equipment and others. Measurement and inspection equipment include scanning electron microscopes, analytical scanning electron microscopes, peripheral equipment and others. Industrial Equipment segment manufactures and sells electron beam lithography equipment, linear electron guns and power supplies and others. Medical Equipment segment manufactures and sells clinical test information processing systems, and automatic amino acid analyzers.