EXO-NET ANZSEV Presentation

For personal use only

Introducing EXO-NET

Next generation exosome isolation

technology

Dr Emily Stein

ANZSEV

10th November 2021

For personal use only

Sample preparation has limited exosome research

Limitations of existing exosome isolation methods:

• Low reproducibility
• Labour intensive
• Time consuming (hours)
• Low throughput (not automatable)
• Yield and purity variable - dependent upon either size or affinity
• Not scalable

Limits translational research and commercialisation of exosome diagnostics and therapeutics

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Introducing EXO-NET®

only
use	Magnetic
personalFor	Bead
Antibodies
	Proprietary linkers

A molecular NET (matrix) comprising eight antibodies (to common external exosome-associated proteins) with spacers and linkers that form a multilayered complex matrix with defined porosity to exclude larger particles.

The EXO-NET matrix is coated onto magnetic beads for ease of use in the laboratory.

EXO-NET:

• Rapid, pure and specific capture of exosomes from any liquid sample (plasma, urine, saliva, culture medium)
• Highly scalable
• Compatible with downstream molecular analyses
• Ideal for high volume clinical laboratory applications.

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For personal use only

EXO-NET simplifies exosome capture

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For personal use only

EXO-NET simplifies exosome capture

Typical research example - Compared to column-basedexosome isolation

solutions (exclusion or affinity chromatography):

96 columns

+

96 tip changes + multiple fraction collectors or centrifuges

++

EXO-NET:	1 microtiter plate +	8 -12 tip changes +	1 magnetic plate

++

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