Holista Colltech Limited announced the independent test results from SGS (Malaysia) Sdn Bhd (SGS) confirming the efficacy of the Company's all-natural Super Bio Nano Silver disinfectant. The Objective of the Test The goal of quantitative suspension tests undertaken by SGS was to evaluate the virucidal activity of the Company's innovation against human coronavirus (ATCC VR-740) and the results showed that Super Bio Nano Silver killed 99.99% of the human coronavirus (ATCC VR-740) after 30 and 60 minutes under the stated condition in accordance with approved European standard EN 14476:2013+A2:2019.1 The standard relates to chemical disinfectants and antiseptics and is a quantitative suspension test for the evaluation of virucidal activity in the medical area. The Testing Period: The test was carried out in accredited labs of SGS in Malaysia between 15 March 2022 and 31 March 2022.

The Test Organism: The test organism used is Human coronavirus, strain 229E, ATCC VR-740. Human coronavirus 229E is propagated in MRC-5 cells (ATCC CCL-171). This strain was isolated from the nasal and throat swabs of a man with a mild upper respiratory illness.

The strain has applications in respiratory disease research and does not result in hemolysis. Super Bio Nano Silver (Silaeris AgNP) killed 99.99% of the human coronavirus (ATCC VR-740) where it was examined at 20°C at the concentration(s) of 150.00ppm [parts per million] for the exposure time(s) of 30 and 60 minutes. After the exposure time(s), the viral reduction exceeded 4 log10-steps in all assays.