Item 8.01 Other Events.

As previously disclosed by Regulus Therapeutics Inc. (the "Company") in the Company's Current Report on Form 8-K filed with the Securities and Exchange Commission (the "SEC") on November 22, 2019, on November 19, 2019 the Company received notice from the Nasdaq Stock Market LLC ("Nasdaq") that the Company's stockholders' equity as reported in the Company's Quarterly Report on Form 10-Q for the quarter ended September 30, 2019 did not satisfy the Nasdaq Capital Market continued listing requirement set forth in Nasdaq Stock Market Rule 5550(b)(1) (the "Stockholders' Equity Requirement").

On December 24, 2019, the Company completed a Milestone Closing under its Securities Purchase Agreement, dated May 3, 2019, with certain institutional and other accredited investors (the "Purchasers"), pursuant to which the Company sold and issued to the Purchasers 3,288,390 shares of newly designated non-voting Class A-2 convertible preferred stock of the Company and accompanying warrants to purchase an aggregate of 32,883,900 shares of common stock of the Company for an aggregate purchase price of approximately $26.0 million. Net proceeds to the Company from the Milestone Closing after deducting placement agent fees but before deducting other expenses payable by the Company are approximately $24.6 million. The Milestone Closing and related matters are described in more detail in the Company's Current Report on Form 8-Kfiled with the SEC on December 24, 2019.

As of the date of this report, the Company believes it has regained compliance with the Stockholders' Equity Requirement based upon the net proceeds to the Company from the Milestone Closing referenced above. Nasdaq will continue to monitor the Company's ongoing compliance with the Stockholders' Equity Requirement and, if at the time of the Company's next periodic report the Company does not evidence compliance with the Stockholders' Equity Requirement, the Company may be subject to delisting. There can be no assurance that the Company will be able to maintain compliance with the Stockholders' Equity Requirement.

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