DIAGNÓSTICOS DA AMÉRICA S.A.

Publicly-Traded Company

NIRE 35.300.172.507

CNPJ/MF n.º 61.486.650/0001-83

MATERIAL FACT

DIAGNÓSTICOS DA AMÉRICA S.A. ("Company" or "DASA"), in attention to CVM Instruction No. 358/03, informs its shareholders and the market that the Company has received, on this date, the attached material fact from Cromossomo Participações II S.A.

Barueri, February 19, 2014.

Paulo Bokel Catta-Preta Chief Investors Relations Officers

Exhibit to the Material Fact dated February 19, 2014

The content of this Exhibit does not constitute a manifestation of Diagnosticos da América S.A.

CROMOSSOMO PARTCIPAçòES Il S.A.

CNPJ!MF Noì4J67.18810001-7ì

N IRE 35.300.411.714

NOTICE TO THE MARKET

So Paulo, 20 2014 CROMOSSOMO PARTICIPAçòES Il S.A.('Cromossomo"). in cornpl1anr:e wllh the sel!orth In !he Brazillan Securllles Comlsslon ("CVM'} Rule No. 358, dated 3 Jar1uary

2002, as amended ('CVM Rule 358) hereby no!ifies da Amèrlca SA. a

company. wtth head office al Avenlda Jaruà, No. 434, Alphavllle, Baruerl, Silo Paulo, CEP 06455-010, enrolled wrrh the CNPJ1MF under No. 61.486.65010001-83 ("Company" or "DASA") that, as a result o!the unffied tender offer of shares !ssued by DASA performed by Cromossomo, pursuant CVM Rule No. 361, dated 5 March 2002 ( cvM Rule 361" and 'OPA'). Cromossomo has become the controlllng sharellolder o! the Company. Thus, the fo!lov:ing mus!be informed:
1. Cromossorno is a (sociedade por Vith head offk:e in the city and state ofSo Paulo. at Rua Joaquim floriano, No. 413, apt 112. llalm Btbì, CEP 04534-011, enrolled wrrh the CNPJIMF under No. 14.167.188ìQOOì-71, lndlrectly controlled by Edson de Godoy Bueno ("Edson") and Dulce
Pugliese de Godoy Bueno {"Dulce"ì;

a. Edson. BrazBian, divorced, physician and businessman, enro!1ed vith the CPFlMF under No.

242.392.247-72, bearer o!the identlty card No. 28.157.352-7, resldent and domlclled In !ha city and state of Rlo Janeìra, wìth office at Avenida das Amérìcas, No. 4200, Edificio MìamL B1oco
7. ala B, ooberi!Jra 302. Barra da Tljuca, CEP 22840-907, and
b. Du1ce, Brazi!ian, divorced. physician, enro!Jed wfth the CPF!MF under No. 242.392247·72, bearer of identity card No. 52.155.62-0. resident and domicJied in the c1ty and state of sao Paulo. wìth off1ce at Rua Haddock Lobo. No. 1725. apt 301, Cerqueira Cesar, CEP 01414-

001.

2. CromossOO'ìo inforrns that, under the OPA. as from the public auction. date, 10 February 2014, unti! the date hereofit ha:s a:cquired 133,726,999 shares, carresponding to 42.89% ofthe total share capftaf of DASA, for a prlce of R$ 15.00 per share. Except far the shares acquired !n the OPA, Cromossamo does not h:ald any oth:er securities canvertible into shares issued by DASA;
3. The alm o!the perforrned by Cromossomo is to ali shares lssued by the DASA;
4. The indlrect contromng sharehotders of Cromossomo. Edson and Du!ce, hold tagether 73,539,384 common shares issued by the Company, which represent 23.59% of the total common shares rssued by DASA Except for those shares of DASA Cromossomo, Edson and Dulce do no!have any other securities or rights convertible in shares of DASA.
5. Thus, an this date, con.sidering the shares lssued by DASA he!d by Cromossomo. together Yith the shares held by !ts fn.direct cantroHin.g shareholders, Edson, Dulce and CrOO'ìassomo, jointly, hofd

Exhibit to the Material Fact dated February 19, 2014

The content of this Exhibit does not constitute a manifestation of Diagnosticos da América S.A.

207,266,383 common shares, representing approximate!y 66.5% of the common shares issued by
DASA;

6. furthermore. v:e inform that on 14 December 2007. Edson and Dulce executed an usufructuary agreement {instrumento partiCJ.Jlar de instituiçào de usufrutoj. as amended on 31 Oe:cember 2007 and on 25 June 2008, through vih;ch Ou!ce granted to Edson the lifelong usufruct of the voting rights of any

equity interest he:à Dulce. v:hich include DASA's shares currentty he!d by Dulce, and any otller rights and securlties underlined by such shares, as 'A'eH as those hat may be acqu!red by Du:ce in the future. Except for the usuffuct mentioned above, no other contract or agreement regulatìng the exercìse of vating rights or the purchase and sale of securit1es issued by DASA has been executed. by Edson, Du!ce or Cro!r':ossomo: and
7. Last!y, Cromossomo states that it v1i:! perform its best efforts to restare the mintmum free float, as required by the Novo Mercado llst!ng segment ru!es of BM&FBOVESPA éLA Bolsa de Valores,
Mercadorìas e Futuros ("Novo Mercado"} and, if such minimum ficat is not restored within six e (six)
months from the conclusion of the OPA, Cromossomo may perform a new tender offer of shares in order to remove DASA from the Navo Mercado.

Sào Paulo, 19 February 2014, PEDRO DE GODOY BUENO

Cramossomo Partlcipaçèìes Il S.A

distributed by