Donaldson Company, Inc. announced its Isolere Bio (Isolere) and Univercells Technologies businesses are developing an integrated upstream and downstream platform for viral vector manufacturing. The first phase will focus on lentivirus (LV) manufacturing, concentrating on improving recoveries with a scalable process while keeping the manufacturing footprint small. Univercells Technologies' scale-X??

fixed-bed bioreactor has demonstrated high yields of infectious LV in perfusion mode. Isolere's IsoTag?? LV affinity purification reagent enables non-chromatographic purification of LV vectors with high functional recoveries, high purity, decreased unit operations and reduced total processing time.

The IsoTag LV reagent confers additional stabilizing properties to the LV vectors that may be advantage when used in conjunction with the scale-X bioreactor. Isolere's pipeline of IsoTag reagents currently is focused on offering affinity purification solutions for adeno-associated virus (AAV), LV and positive-strand RNA viruses (ssRNA). These reagents combine tailored affinity ligands with liquid-liquid phase separation to sequester target biologics from complex solutions into large droplets.

These droplets are concentrated and purified via tangential flow filtration using familiar equipment and off-the-shelf consumables.